Abstract
Rx is a homeobox-containing gene that is critical for vertebrate eye development. Its expression domain delineates a field of cells from which the retina and the ventral hypothalamus develop. The 5' upstream regulatory sequences of the medaka fish Rx gene are functionally conserved during evolution to a degree that they direct gene expression into the Rx-expressing field of cells in mice. Using these sequences, we made a Cre line that can be used for inactivation of gene expression in the developing retina.
Publication types
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Letter
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Research Support, N.I.H., Extramural
MeSH terms
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Alleles
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Animals
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Eye Proteins / genetics*
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Eye Proteins / physiology*
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Gene Expression Regulation, Developmental*
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Genes, Homeobox*
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Genes, Reporter
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Green Fluorescent Proteins / metabolism
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Homeodomain Proteins / genetics*
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Homeodomain Proteins / physiology*
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Integrases / genetics
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Lac Operon
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Mice
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Mice, Transgenic
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Oryzias / embryology
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Oryzias / genetics
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Retina / embryology*
Substances
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Eye Proteins
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Homeodomain Proteins
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Rx protein, mouse
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Green Fluorescent Proteins
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Cre recombinase
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Integrases