Selective detection and transport of fully matched DNA by DNA-loaded microtubule and kinesin motor protein

Shu Taira; Yong-Zhong Du; Yuichi Hiratsuka; Kaoru Konishi; Tai Kubo; Taro Q P Uyeda; Noboru Yumoto; Masato Kodaka

doi:10.1002/bit.21055

Selective detection and transport of fully matched DNA by DNA-loaded microtubule and kinesin motor protein

Biotechnol Bioeng. 2006 Oct 20;95(3):533-8. doi: 10.1002/bit.21055.

Authors

Shu Taira¹, Yong-Zhong Du, Yuichi Hiratsuka, Kaoru Konishi, Tai Kubo, Taro Q P Uyeda, Noboru Yumoto, Masato Kodaka

Affiliation

¹ Institute for Biological Resources and Functions, National Institute of Advanced Industrial Science and Technology, AIST, Central 6, 1-1-1 Higashi, Tsukuba 305-8566, Japan.

PMID: 16897728
DOI: 10.1002/bit.21055

Abstract

DNA-loaded microtubules (MTs) moving on a kinesin motor protein-coated substrate can selectively hybridize with a target fully matched DNA over single-base mismatched DNA and transport it. This technique is capable of collecting target biomolecules toward one point site to design new methodology of DNA analysis.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Adsorption
Animals
Bacterial Proteins / chemistry*
Biotin / analogs & derivatives*
Biotin / chemistry
DNA / isolation & purification*
DNA Probes*
Kinesins / chemistry*
Microscopy, Fluorescence
Microtubule-Associated Proteins / chemistry*
Swine

Substances

Bacterial Proteins
DNA Probes
Microtubule-Associated Proteins
biotin-streptavidin complex
Biotin
DNA
Kinesins