Abstract
We present a thorough introduction into the recently developed fluorescence lifetime correlation spectroscopy (FLCS). The theoretical basis of FLCS is explained, and the method is applied to the study of a dynamic transition between two fluorescence lifetime states in a dye-protein complex.
MeSH terms
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Biophysical Phenomena
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Biophysics
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Carbocyanines / chemistry*
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Fluorescent Dyes / chemistry*
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Models, Chemical*
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Molecular Conformation
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Protein Conformation
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Spectrometry, Fluorescence / methods*
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Streptavidin / chemistry*
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Time Factors
Substances
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Carbocyanines
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Fluorescent Dyes
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cyanine dye 5
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Streptavidin