Abstract
A significant level of target degradation was caused by bisulfite treatment for methylcytosine-selective hydrolysis. The depyrimidination proceeded via addition of bisulfite to pyrimidines in DNA. The quantification with real-time PCR after conventional bisulfite treatment showed a large decrease in the amount of full-length DNA.
MeSH terms
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Absorption
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Chemistry, Pharmaceutical / methods*
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Cytosine / chemistry
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DNA / chemistry*
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Drug Design
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Epigenesis, Genetic
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Gene Expression
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Models, Chemical
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Molecular Conformation
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Polymerase Chain Reaction
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Polymers / chemistry
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Reverse Transcriptase Polymerase Chain Reaction
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Sulfites / chemistry*
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Time Factors
Substances
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Polymers
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Sulfites
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Cytosine
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DNA
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hydrogen sulfite