Chz1, a nuclear chaperone for histone H2AZ

Ed Luk; Ngoc-Diep Vu; Kem Patteson; Gaku Mizuguchi; Wei-Hua Wu; Anand Ranjan; Jonathon Backus; Subhojit Sen; Marc Lewis; Yawen Bai; Carl Wu

doi:10.1016/j.molcel.2006.12.015

Chz1, a nuclear chaperone for histone H2AZ

Mol Cell. 2007 Feb 9;25(3):357-68. doi: 10.1016/j.molcel.2006.12.015.

Authors

Ed Luk¹, Ngoc-Diep Vu, Kem Patteson, Gaku Mizuguchi, Wei-Hua Wu, Anand Ranjan, Jonathon Backus, Subhojit Sen, Marc Lewis, Yawen Bai, Carl Wu

Affiliation

¹ Laboratory of Biochemistry and Molecular Biology, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA. luked@mail.nih.gov

PMID: 17289584
DOI: 10.1016/j.molcel.2006.12.015

Abstract

The histone variant H2AZ marks nucleosomes flanking the promoters of most genes of budding yeast. The incorporation of H2AZ into chromatin is dependent on the SWR1 complex, which catalyses the replacement of conventional histone H2A with H2AZ. In cells, the pool of unincorporated histone H2AZ has previously been found in association with Nap1, a chaperone for conventional histone H2A-H2B. Here, we report the discovery of Chz1, a histone chaperone that has preference for H2AZ and can also deliver a source of the histone variant for SWR1-dependent histone replacement. Bacterially expressed Chz1 forms a heterotrimer with H2AZ-H2B, stabilizing the association of the histone dimer. We have identified a conserved motif important for histone variant recognition within the H2AZ-interacting domain of Chz1. The presence of this motif in other metazoan proteins suggests that H2AZ-specific chaperones may be widely conserved.

Publication types

Research Support, N.I.H., Intramural

MeSH terms

Adenosine Triphosphatases / metabolism
Amino Acid Sequence
Carrier Proteins / chemistry
Cell Cycle Proteins / physiology
Conserved Sequence
Dimerization
Histone Chaperones
Histones / metabolism*
Humans
Molecular Chaperones / chemistry
Molecular Chaperones / metabolism
Molecular Chaperones / physiology*
Molecular Sequence Data
Nuclear Proteins / physiology
Nucleosome Assembly Protein 1
Saccharomyces cerevisiae / genetics
Saccharomyces cerevisiae / metabolism*
Saccharomyces cerevisiae Proteins / chemistry
Saccharomyces cerevisiae Proteins / metabolism
Saccharomyces cerevisiae Proteins / physiology*
Sequence Alignment

Substances

Carrier Proteins
Cell Cycle Proteins
Chz1 protein, S cerevisiae
HIRIP3 protein, human
Histone Chaperones
Histones
Htz1 protein, S cerevisiae
Molecular Chaperones
NAP1 protein, S cerevisiae
NAP1L1 protein, human
Nuclear Proteins
Nucleosome Assembly Protein 1
Saccharomyces cerevisiae Proteins
Adenosine Triphosphatases
Swr1 protein, S cerevisiae

Grants and funding

Intramural NIH HHS/United States