An overview is given of the role of Malpighian (renal) tubules and the hindgut (ileum and rectum) in the excretory process of insects. The review focuses on the mechanism of primary urine production by Malpighian tubules and its control by neurohormones, which includes serotonin and neuropeptides resembling mammalian corticotropin-releasing factor (CRF) and calcitonin. Particular emphasis is given to in vitro studies of the effect of neurohormones on Malpighian tubule ion transport and a consideration of their likely role in the regulation of salt balance in vivo.