This study examined the sensitivity of four cochlear implant (CI) listeners to interaural time difference (ITD) in different portions of four-pulse sequences in lateralization discrimination. ITD was present either in all the pulses (referred to as condition Wave), the two middle pulses (Ongoing), the first pulse (Onset), the last pulse (Offset), or both the first and last pulse (Gating). All ITD conditions were tested at different pulse rates (100, 200, 400, and 800 pulses/s pps). Also, five normal hearing (NH) subjects were tested, listening to an acoustic simulation of CI stimulation. All CI and NH listeners were sensitive in condition Gating at all pulse rates for which they showed sensitivity in condition Wave. The sensitivity in condition Onset increased with the pulse rate for three CI listeners as well as for all NH listeners. The performance in condition Ongoing varied over the subjects. One CI listener showed sensitivity up to 800 pps, two up to 400 pps, and one at 100 pps only. The group of NH listeners showed sensitivity up to 200 pps. The result that CI listeners detect ITD from the middle pulses of short trains indicates the relevance of fine timing of stimulation pulses in lateralization and therefore in CI stimulation strategies.