Abstract
In situ hybridization techniques typically employ chromogenic staining by enzymatic amplification to detect domains of gene expression. We demonstrate the previously unreported near infrared (NIR) fluorescence of the dark purple stain formed from the commonly used chromogens, nitro blue tetrazolium (NBT) and 5-bromo-4-chloro-3-indolyl phosphate (BCIP). The solid reaction product has significant fluorescence that enables the use of confocal microscopy to generate high-resolution three-dimensional (3-D) imaging of gene expression.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, Non-P.H.S.
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Technical Report
MeSH terms
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Animals
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Cardiac Myosins / genetics
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Cardiac Myosins / metabolism
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Embryo, Nonmammalian
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In Situ Hybridization, Fluorescence / methods*
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Indoles / chemistry*
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Lampreys / embryology
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Microscopy, Confocal
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Nitroblue Tetrazolium / chemistry*
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Staining and Labeling*
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Zebrafish / embryology
Substances
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Indoles
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Nitroblue Tetrazolium
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5-bromo-4-chloro-3-indoxyl phosphate
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Cardiac Myosins