Abstract
We developed a general method, microarray-based genomic selection (MGS), capable of selecting and enriching targeted sequences from complex eukaryotic genomes without the repeat blocking steps necessary for bacterial artificial chromosome (BAC)-based genomic selection. We demonstrate that large human genomic regions, on the order of hundreds of kilobases, can be enriched and resequenced with resequencing arrays. MGS, when combined with a next-generation resequencing technology, can enable large-scale resequencing in single-investigator laboratories.
Publication types
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Research Support, N.I.H., Extramural
MeSH terms
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Animals
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Eukaryotic Cells / metabolism
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Fragile X Mental Retardation Protein / genetics
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Gene Library
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Genome / genetics*
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Genome, Human / genetics
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Humans
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Mutation
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Nuclear Proteins / genetics
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Nucleic Acid Hybridization
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Oligodeoxyribonucleotides / genetics
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Oligonucleotide Array Sequence Analysis / methods*
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Polymerase Chain Reaction
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Reproducibility of Results
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Sequence Analysis, DNA / methods*
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Trans-Activators / genetics
Substances
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AFF2 protein, human
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FMR1 protein, human
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Nuclear Proteins
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Oligodeoxyribonucleotides
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Trans-Activators
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Fragile X Mental Retardation Protein