Abstract
We have developed the first selective fluorescent chemosensor (PyDPA) for (p)ppGpp, a bacterial and plant alarmone. By using pyrene-excimer fluorescence, PyDPA shows very good selectivity for (p)ppGpp from among other nucleotides in water. PyDPA was used for the real-time detection of in vitro ppGpp synthesis by bacterial ribosomal complexes.
Publication types
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Research Support, N.I.H., Intramural
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Research Support, Non-U.S. Gov't
MeSH terms
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Bacteria / metabolism*
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Fluorescence
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Fluorescent Dyes / chemistry*
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Guanosine Pentaphosphate / analysis*
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Pyrenes / chemistry*
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Pyridines / chemistry*
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Water / chemistry
Substances
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(1-((pyren-1-yl)propoxy)-3,5-bis(bis(2-pyridylmethyl)aminomethyl)benzene)zinc(II)
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Fluorescent Dyes
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Pyrenes
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Pyridines
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Water
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Guanosine Pentaphosphate