Oral immunization of broiler chickens against necrotic enteritis with an attenuated Salmonella vaccine vector expressing Clostridium perfringens antigens

R R Kulkarni; V R Parreira; S Sharif; J F Prescott

doi:10.1016/j.vaccine.2008.05.079

Oral immunization of broiler chickens against necrotic enteritis with an attenuated Salmonella vaccine vector expressing Clostridium perfringens antigens

Vaccine. 2008 Aug 5;26(33):4194-203. doi: 10.1016/j.vaccine.2008.05.079. Epub 2008 Jun 17.

Authors

R R Kulkarni¹, V R Parreira, S Sharif, J F Prescott

Affiliation

¹ Department of Pathobiology, University of Guelph, Guelph, Ontario N1G 2W1, Canada.

PMID: 18597901
DOI: 10.1016/j.vaccine.2008.05.079

Abstract

Necrotic enteritis (NE) in broiler chickens is caused by Clostridium perfringens but currently no effective vaccine is available. Our previous study showed that certain C. perfringens secreted proteins when administered intramuscularly protected chickens against experimental infection. In the current study, genes encoding three C. perfringens proteins: fructose-biphosphate-aldolase (FBA), pyruvate:ferredoxin-oxidoreductase (PFOR) and hypothetical protein (HP), were cloned into an avirulent Salmonella enterica sv. typhimurium vaccine vector. Broiler chickens immunized orally with recombinant Salmonella expressing FBA or HP proteins were significantly protected against NE challenge. Immunized birds developed serum and mucosal antibodies to both clostridial and Salmonella antigens. This study showed the oral immunizing ability of two C. perfringens antigens against NE in broiler chickens through an attenuated Salmonella vaccine vector.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Administration, Oral
Animals
Antibodies, Bacterial / analysis
Antibodies, Bacterial / blood
Antigens, Bacterial / genetics
Antigens, Bacterial / immunology
Bacterial Proteins / genetics
Bacterial Proteins / immunology
Body Weight
Chickens
Clostridium Infections / prevention & control*
Clostridium perfringens / genetics
Clostridium perfringens / immunology
Enteritis / prevention & control
Enteritis / veterinary*
Fructose-Bisphosphate Aldolase / genetics
Fructose-Bisphosphate Aldolase / immunology
Intestines / immunology
Intestines / pathology
Poultry Diseases / prevention & control*
Pyruvate Synthase / genetics
Pyruvate Synthase / immunology
Salmonella Vaccines / immunology*
Salmonella typhimurium / genetics
Vaccines, Attenuated / immunology
Vaccines, Synthetic / immunology

Substances

Antibodies, Bacterial
Antigens, Bacterial
Bacterial Proteins
Salmonella Vaccines
Vaccines, Attenuated
Vaccines, Synthetic
Pyruvate Synthase
Fructose-Bisphosphate Aldolase