Application of lipase to concentrate the docosahexaenoic acid (DHA) fraction of fish oil

V B Yadwad; O P Ward; L C Noronha

doi:10.1002/bit.260380818

Application of lipase to concentrate the docosahexaenoic acid (DHA) fraction of fish oil

Biotechnol Bioeng. 1991 Oct 20;38(8):956-9. doi: 10.1002/bit.260380818.

Authors

V B Yadwad¹, O P Ward, L C Noronha

Affiliation

¹ Biology Department, University of Waterloo, Waterloo, Ontario, Canada.

PMID: 18600853
DOI: 10.1002/bit.260380818

Abstract

A commercial lipase preparation from Rhizopus niveus was used to concentrate the omega-3 fatty acid, docosahexaenoic acid (DHA) component in fish oil. The DHA content of cod-liver oil was 9.64% (w/w) of total fatty acids. Enzymatic digestion conditions were established which produced a DHA content in the monoglycerides fraction of 29.17% (w/w) of total fatty acid, triglyceride, and diglyceride components were 5.72, 9.95, and 15316%, respectively.