Abstract
Cells regulate gene expression using a complex network of signaling pathways, transcription factors and promoters. To gain insight into the structure and function of these networks, we analyzed gene expression in single- and multiple-mutant strains to build a quantitative model of the Hog1 MAPK-dependent osmotic stress response in budding yeast. Our model reveals that the Hog1 and general stress (Msn2/4) pathways interact, at both the signaling and promoter level, to integrate information and create a context-dependent response. This study lays out a path to identifying and characterizing the role of signal integration and processing in other gene regulatory networks.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Binding Sites
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Chromatin Immunoprecipitation
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Enzyme Activation
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Gene Expression Regulation, Fungal
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Gene Regulatory Networks*
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Mitogen-Activated Protein Kinases / chemistry
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Mitogen-Activated Protein Kinases / metabolism*
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Models, Biological
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Mutation / genetics
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Osmotic Pressure
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Saccharomyces cerevisiae / enzymology*
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Saccharomyces cerevisiae / genetics*
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Saccharomyces cerevisiae Proteins / chemistry
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Saccharomyces cerevisiae Proteins / genetics
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Saccharomyces cerevisiae Proteins / metabolism*
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Signal Transduction
Substances
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Saccharomyces cerevisiae Proteins
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HOG1 protein, S cerevisiae
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Mitogen-Activated Protein Kinases