Abstract
We present the instrumental development of a versatile total internal reflection fluorescence lifetime imaging microscopy setup illuminated by a supercontinuum laser source. It enables performing wide-field fluorescence lifetime imaging with subwavelength axial resolution for a large range of fluorophores. The short overall acquisition time and the axial resolution are well suited for dynamic neurobiological applications.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Cell Line
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Equipment Design
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Fluorescence
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Fluorescence Resonance Energy Transfer / methods
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Fluorescent Dyes / pharmacology
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Green Fluorescent Proteins / metabolism
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Humans
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Lasers
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Microscopy, Fluorescence / instrumentation*
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Microscopy, Fluorescence / methods*
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Neurobiology / methods
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Optics and Photonics*
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Protein Interaction Mapping
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Refractometry / methods
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Reproducibility of Results
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Time Factors
Substances
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Cyan Fluorescent Protein
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Fluorescent Dyes
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Green Fluorescent Proteins