Extending the range of microsecond-to-millisecond chemical exchange detected in labeled and unlabeled nucleic acids by selective carbon R(1rho) NMR spectroscopy

Alexandar L Hansen; Evgenia N Nikolova; Anette Casiano-Negroni; Hashim M Al-Hashimi

doi:10.1021/ja8091399

Extending the range of microsecond-to-millisecond chemical exchange detected in labeled and unlabeled nucleic acids by selective carbon R(1rho) NMR spectroscopy

J Am Chem Soc. 2009 Mar 25;131(11):3818-9. doi: 10.1021/ja8091399.

Authors

Alexandar L Hansen¹, Evgenia N Nikolova, Anette Casiano-Negroni, Hashim M Al-Hashimi

Affiliation

¹ Department of Chemistry and Biophysics, University of Michigan, 930 North University Avenue, Ann Arbor, Michigan 48109, USA.

PMID: 19243182
DOI: 10.1021/ja8091399

Abstract

We present an off-resonance carbon R(1rho) NMR experiment utilizing weak radiofrequency fields and selective polarization transfers for quantifying chemical-exchange processes in nucleic acids. The experiment extends the range of accessible time scales to approximately 10 ms, and its time-saving feature makes it possible to thoroughly map out dispersion profiles and conduct measurements at natural abundance. The experiment unveiled microsecond-to-millisecond exchange dynamics in a uniformly labeled A-site rRNA and in unlabeled, damaged DNA that would otherwise be difficult to characterize by conventional methods.

Publication types

Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

Carbon Isotopes
DNA / chemistry
DNA Damage
Kinetics
Magnetic Resonance Spectroscopy / instrumentation
Magnetic Resonance Spectroscopy / methods*
Nucleic Acids / chemistry*
RNA, Ribosomal / chemistry

Substances

Carbon Isotopes
Nucleic Acids
RNA, Ribosomal
DNA