Abstract
Satellite cells are the resident stem cells of adult skeletal muscle. To date though, there is a paucity of native markers that can be used to easily identify quiescent satellite cells, with Pax7 probably being the best that is currently available. Here we have further characterized a number of recently described satellite cell markers, and also describe novel ones. Caveolin-1, integrin alpha7 and the calcitonin receptor proved reliable markers for quiescent satellite cells, being expressed by all satellite cells identified with Pax7. These three markers remained expressed as satellite cells were activated and underwent proliferation. The nuclear envelope proteins lamin A/C and emerin, mutations in which underlie Emery-Dreifuss muscular dystrophy, were also expressed in both quiescent and proliferating satellite cells. Conversely, Jagged-1, a Notch ligand, was not expressed in quiescent satellite cells but was induced upon activation. These findings further contribute to defining the molecular signature of muscle satellite cells.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Antigens, CD / genetics
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Antigens, CD / metabolism
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Calcium-Binding Proteins / genetics
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Calcium-Binding Proteins / metabolism
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Caveolin 1 / genetics
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Caveolin 1 / metabolism
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Cell Differentiation / genetics
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Cell Differentiation / physiology
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Gene Expression
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Gene Expression Profiling*
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Integrin alpha Chains / genetics
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Integrin alpha Chains / metabolism
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Intercellular Signaling Peptides and Proteins / genetics
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Intercellular Signaling Peptides and Proteins / metabolism
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Jagged-1 Protein
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Lamin Type A / genetics
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Lamin Type A / metabolism
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Membrane Proteins / genetics
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Membrane Proteins / metabolism
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Mice
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Mice, Inbred C57BL
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Nuclear Proteins / genetics
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Nuclear Proteins / metabolism
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PAX7 Transcription Factor / genetics
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PAX7 Transcription Factor / metabolism
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RNA, Messenger / metabolism
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Receptors, Calcitonin / genetics
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Receptors, Calcitonin / metabolism
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Satellite Cells, Skeletal Muscle / metabolism*
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Satellite Cells, Skeletal Muscle / physiology
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Serrate-Jagged Proteins
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Stem Cells / metabolism*
Substances
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Antigens, CD
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Calcium-Binding Proteins
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Caveolin 1
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Integrin alpha Chains
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Intercellular Signaling Peptides and Proteins
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JAG1 protein, human
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Jag1 protein, mouse
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Jagged-1 Protein
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Lamin Type A
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Membrane Proteins
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Nuclear Proteins
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PAX7 Transcription Factor
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PAX7 protein, human
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RNA, Messenger
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Receptors, Calcitonin
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Serrate-Jagged Proteins
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emerin
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integrin alpha7