Abstract
A lipophilic dye based on the Bodipy fluorophore, LD540, was developed for microscopic imaging of lipid droplets. In contrast to previous lipid droplet dyes, it can spectrally be resolved from both green and red fluorophores allowing multicolor imaging in both fixed and living cells. Its improved specificity, brightness and photostability support live cell imaging, which was used to demonstrate by two-color imaging lipid droplet motility along microtubules.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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3T3-L1 Cells
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Adipocytes / chemistry*
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Animals
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COS Cells
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Carcinoma, Hepatocellular / pathology
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Carrier Proteins / metabolism
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Cell Line, Tumor
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Chlorocebus aethiops
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Color
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Fluorescent Dyes / chemical synthesis
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Fluorescent Dyes / chemistry
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Fluorescent Dyes / metabolism*
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Green Fluorescent Proteins / metabolism
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Humans
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Lipids / analysis*
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Liver Neoplasms / pathology
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Membrane Proteins
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Mice
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Microscopy, Confocal
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Microtubules / metabolism
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Sensitivity and Specificity
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Spectrometry, Fluorescence
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Transfection
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Tubulin / metabolism
Substances
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AUP1 protein, human
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Carrier Proteins
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Fluorescent Dyes
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Lipids
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Membrane Proteins
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Tubulin
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enhanced green fluorescent protein
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Green Fluorescent Proteins