Using Schizosaccharomyces pombe meiosis to analyze DNA recombination intermediates

Methods Mol Biol. 2009:557:235-52. doi: 10.1007/978-1-59745-527-5_15.

Abstract

The fission yeast Schizosaccharomyces pombe has many biological characteristics that make it an ideal model organism for the study of meiosis. A nearly synchronous meiosis is one of the most important. Under certain environmental and genetic conditions, large cultures of S. pombe can be induced to undergo meiosis in a timely and predictable manner that allows for changes in the DNA to be observed and analyzed by gel electrophoresis. Initiation of meiotic recombination via programmed DNA double-strand breaks, the formation of joint molecule recombination intermediates, and the resolution of these intermediates into crossover DNA products can all be seen with consistent timing during the progression of a synchronous meiotic induction. The timing of recombination events, the genetic requirements for the formation and disappearance of recombination intermediates, and the analysis of the DNA structures of those intermediates allow a comparison of meiotic recombination in fission yeast with that in the only other species similarly studied, the budding yeast Saccharomyces cerevisiae.

Publication types

  • Research Support, N.I.H., Extramural
  • Review

MeSH terms

  • Cell Culture Techniques / methods
  • DNA, Fungal / analysis*
  • DNA, Fungal / chemistry
  • DNA, Fungal / isolation & purification
  • Electrophoresis, Agar Gel / methods
  • Meiosis / genetics*
  • Models, Biological
  • Recombination, Genetic / genetics*
  • Schizosaccharomyces / chemistry
  • Schizosaccharomyces / genetics*
  • Schizosaccharomyces / growth & development

Substances

  • DNA, Fungal