Abstract
The slow kinetics and low efficiency of reprogramming methods to generate human induced pluripotent stem cells (iPSCs) impose major limitations on their utility in biomedical applications. Here we describe a chemical approach that dramatically improves (200-fold) the efficiency of iPSC generation from human fibroblasts, within seven days of treatment. This will provide a basis for developing safer, more efficient, nonviral methods for reprogramming human somatic cells.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, Non-U.S. Gov't
MeSH terms
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Benzamides / pharmacology
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Cell Differentiation / genetics*
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Dioxoles / pharmacology
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Diphenylamine / analogs & derivatives
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Diphenylamine / pharmacology
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Fibroblasts / physiology
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Humans
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Induced Pluripotent Stem Cells / cytology*
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Induced Pluripotent Stem Cells / physiology
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MAP Kinase Kinase 1 / antagonists & inhibitors
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Pyrimidines / pharmacology
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Receptors, Transforming Growth Factor beta / antagonists & inhibitors
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Thiazoles / pharmacology
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Transduction, Genetic
Substances
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4-(5-benzo(1,3)dioxol-5-yl-4-pyridin-2-yl-1H-imidazol-2-yl)benzamide
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Benzamides
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Dioxoles
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Pyrimidines
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Receptors, Transforming Growth Factor beta
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Thiazoles
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thiazovivin
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mirdametinib
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Diphenylamine
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MAP Kinase Kinase 1