Abstract
We report transgenic mouse models in which three or four reprogramming factors are expressed from a single genomic locus using a drug-inducible transgene. Multiple somatic cell types can be directly reprogrammed to generate induced pluripotent stem cells (iPSCs) by culture in doxycycline. Because reprogramming factors are carried on a single polycistronic construct, the mice can be easily maintained, and the transgene can be easily transferred into other genetic backgrounds.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Cell Dedifferentiation / drug effects
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Cells, Cultured
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Cellular Reprogramming / drug effects
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Cellular Reprogramming / genetics*
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Doxycycline / pharmacology
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Gene Expression Regulation / drug effects
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Genome
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Induced Pluripotent Stem Cells / cytology*
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Induced Pluripotent Stem Cells / drug effects
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Induced Pluripotent Stem Cells / metabolism*
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Integrases / metabolism
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Mice
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Mice, Transgenic
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Mutagenesis, Insertional
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Organ Specificity
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Recombination, Genetic
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Transgenes / genetics*
Substances
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Cre recombinase
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Integrases
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Doxycycline