Abstract
The derivation of induced pluripotent stem cells (iPSCs) usually involves the viral introduction of reprogramming factors into somatic cells. Here we used gene targeting to generate a mouse strain with a single copy of an inducible, polycistronic reprogramming cassette, allowing for the induction of pluripotency in various somatic cell types. As these 'reprogrammable mice' can be easily bred, they are a useful tool to study the mechanisms underlying cellular reprogramming.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Blood Cells / cytology
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Blood Cells / drug effects
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Cell Dedifferentiation / drug effects
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Cell Lineage / drug effects
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Cell Lineage / genetics
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Cells, Cultured
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Cellular Reprogramming / drug effects
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Cellular Reprogramming / genetics*
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Collagen / genetics
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DNA Nucleotidyltransferases / genetics
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DNA Nucleotidyltransferases / metabolism
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Doxycycline / pharmacology
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Embryonic Stem Cells / cytology*
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Embryonic Stem Cells / drug effects
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Embryonic Stem Cells / metabolism
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Fibroblasts / cytology
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Fibroblasts / drug effects
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Fibroblasts / metabolism
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Gene Targeting*
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Induced Pluripotent Stem Cells / cytology*
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Induced Pluripotent Stem Cells / drug effects
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Induced Pluripotent Stem Cells / metabolism
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Mice
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Mice, Mutant Strains
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Mutagenesis, Insertional / genetics
Substances
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Collagen
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DNA Nucleotidyltransferases
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FLP recombinase
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Doxycycline