Abstract
A protein closely related to SV40 T antigen was purified in a biologically active form from cells infected with the defective adenovirus-SV40 hybrid, Ad2+D2. This 107,000 dalton hybrid protein binds and protects a specific portion of SV40 DNA from digestion by pancreatic DNAase I. Hybridization, endonuclease cleavage and pyrimidine tract analysis of the protected fragments reveal that the D2 hybrid protein binds in a sequential manner to tandem recognition sites which lie within a sequence of 120 nucleotides at position 67 near the origin of SV40 replication.
Publication types
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Adenoviridae
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Antigens, Neoplasm*
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Antigens, Viral*
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Base Sequence
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Binding Sites
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DNA Replication
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DNA, Viral / metabolism*
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Defective Viruses
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Deoxyribonucleases / metabolism
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Simian virus 40* / immunology
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Viral Proteins / isolation & purification
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Viral Proteins / metabolism*
Substances
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Antigens, Neoplasm
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Antigens, Viral
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DNA, Viral
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Viral Proteins
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Deoxyribonucleases
Associated data
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GENBANK/J02400
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GENBANK/J02402
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GENBANK/J02403
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GENBANK/J02406
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GENBANK/J02407
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GENBANK/J02408
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GENBANK/J02409
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GENBANK/J02410
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GENBANK/V01380