High rates of homologous recombination in the mite endosymbiont and opportunistic human pathogen Orientia tsutsugamushi

PLoS Negl Trop Dis. 2010 Jul 20;4(7):e752. doi: 10.1371/journal.pntd.0000752.

Abstract

Orientia tsutsugamushi is an intracellular alpha-proteobacterium which resides in trombiculid mites, and is the causative agent of scrub typhus in East Asia. The genome sequence of this species has revealed an unprecedented number of repeat sequences, most notably of the genes encoding the conjugative properties of a type IV secretion system (T4SS). Although this observation is consistent with frequent intragenomic recombination, the extent of homologous recombination (gene conversion) in this species is unknown. To address this question, and to provide a protocol for the epidemiological surveillance of this important pathogen, we have developed a multilocus sequence typing (MLST) scheme based on 7 housekeeping genes (gpsA, mdh, nrdB, nuoF, ppdK, sucD, sucB). We applied this scheme to the two published genomes, and to DNA extracted from blood taken from 84 Thai scrub typhus patients, from 20 cultured Thai patient isolates, 1 Australian patient sample, and from 3 cultured type strains. These data demonstrated that the O. tsutsugamushi population was both highly diverse [Simpson's index (95% CI) = 0.95 (0.92-0.98)], and highly recombinogenic. These results are surprising given the intracellular life-style of this species, but are broadly consistent with results obtained for Wolbachia, which is an alpha-proteobacterial reproductive parasite of arthropods. We also compared the MLST data with ompA sequence data and noted low levels of consistency and much higher discrimination by MLST. Finally, twenty-five percent of patients in this study were simultaneously infected with multiple sequence types, suggesting multiple infection caused by either multiple mite bites, or multiple strains co-existing within individual mites.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Bacterial Typing Techniques / methods
  • DNA Fingerprinting / methods
  • DNA, Bacterial / chemistry
  • DNA, Bacterial / genetics
  • Humans
  • Mites / microbiology
  • Orientia tsutsugamushi / genetics*
  • Orientia tsutsugamushi / isolation & purification
  • Polymorphism, Genetic*
  • Recombination, Genetic*
  • Scrub Typhus / microbiology
  • Sequence Analysis, DNA / methods

Substances

  • DNA, Bacterial