Abstract
Despite the mitochondria ubiquitous nature many of their components display divergences in their expression profile across different tissues. Using the bioinformatics-approach of guilt by association (GBA) we exploited these variations to predict the function of two so far poorly annotated genes: Coiled-coil-helix-coiled-coil-helix domain containing 10 (CHCHD10) and glioblastoma amplified sequence (GBAS). We predicted both genes to be involved in oxidative phosphorylation. Through in vitro experiments using gene-knockdown we could indeed confirm this and furthermore we asserted CHCHD10 to play a role in complex IV activity.
Copyright © 2010. Published by Elsevier Inc.
MeSH terms
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Adenosine Triphosphate / biosynthesis*
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Computational Biology / methods
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Electron Transport Complex IV / metabolism*
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Gene Knockdown Techniques
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Genes, Mitochondrial / physiology*
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HeLa Cells
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Heart / physiology*
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Humans
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Intracellular Signaling Peptides and Proteins
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Membrane Proteins / genetics
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Membrane Proteins / physiology*
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Mitochondrial Proteins / genetics
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Mitochondrial Proteins / physiology*
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Oxidative Phosphorylation*
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Phosphoproteins / genetics
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Phosphoproteins / physiology*
Substances
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CHCHD10 protein, human
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Intracellular Signaling Peptides and Proteins
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Membrane Proteins
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Mitochondrial Proteins
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NIPSNAP2 protein, human
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Phosphoproteins
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Adenosine Triphosphate
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Electron Transport Complex IV