Abstract
Dental caries is initiated by demineralization of the tooth surface through acid production by sugar metabolism of supragingival plaque microflora. To elucidate the sugar metabolic system, we used CE-MS to perform metabolomics of the central carbon metabolism, the EMP pathway, the pentose-phosphate pathway, and the TCA cycle in supra- gingival plaque and representative oral bacteria, Streptococcus and Actinomyces. Supragingival plaque contained all the targeted metabolites in the central carbon metabolism, except erythrose 4-phosphate in the pentose-phosphate pathway. After glucose rinse, glucose 6-phosphate, fructose 6-phosphate, fructose 1,6-bisphosphate, dihydroxyacetone phosphate, and pyruvate in the EMP pathway and 6-phosphogluconate, ribulose 5-phosphate, and sedoheptulose 7-phosphate in the pentose-phosphate pathway, and acetyl CoA were increased. Meanwhile, 3-phosphoglycerate and phosphoenolpyruvate in the EMP pathway and succinate, fumarate, and malate in the TCA cycle were decreased. These pathways and changes in metabolites observed in supragingival plaque were similar to the integration of metabolite profiles in Streptococcus and Actinomyces.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Acetyl Coenzyme A / analysis
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Actinomyces / classification
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Actinomyces / metabolism*
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Adult
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Bacteriological Techniques
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Carbon / metabolism
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Citric Acid Cycle / physiology
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Dental Plaque / microbiology*
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Dihydroxyacetone Phosphate / analysis
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Female
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Fructosediphosphates / analysis
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Fructosephosphates / analysis
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Fumarates / analysis
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Gluconates / analysis
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Glucose / metabolism
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Glucose-6-Phosphate / analysis
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Glyceric Acids / analysis
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Glycolysis / physiology
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Humans
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Malates / analysis
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Male
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Metabolomics*
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Pentose Phosphate Pathway / physiology
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Phosphoenolpyruvate / analysis
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Pyruvic Acid / analysis
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Ribulosephosphates / analysis
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Streptococcus / classification
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Streptococcus / metabolism*
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Streptococcus mutans / metabolism
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Succinic Acid / analysis
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Sugar Phosphates / analysis
Substances
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Fructosediphosphates
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Fructosephosphates
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Fumarates
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Gluconates
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Glyceric Acids
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Malates
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Ribulosephosphates
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Sugar Phosphates
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sedoheptulose 7-phosphate
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ribulose 5-phosphate
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Glucose-6-Phosphate
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Dihydroxyacetone Phosphate
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Acetyl Coenzyme A
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Phosphoenolpyruvate
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Carbon
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malic acid
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3-phosphoglycerate
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Pyruvic Acid
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fumaric acid
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Succinic Acid
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Glucose
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fructose-1,6-diphosphate
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6-phosphogluconic acid