Abstract
Jasmonates are a family of plant hormones that regulate plant growth, development and responses to stress. The F-box protein CORONATINE INSENSITIVE 1 (COI1) mediates jasmonate signalling by promoting hormone-dependent ubiquitylation and degradation of transcriptional repressor JAZ proteins. Despite its importance, the mechanism of jasmonate perception remains unclear. Here we present structural and pharmacological data to show that the true Arabidopsis jasmonate receptor is a complex of both COI1 and JAZ. COI1 contains an open pocket that recognizes the bioactive hormone (3R,7S)-jasmonoyl-l-isoleucine (JA-Ile) with high specificity. High-affinity hormone binding requires a bipartite JAZ degron sequence consisting of a conserved α-helix for COI1 docking and a loop region to trap the hormone in its binding pocket. In addition, we identify a third critical component of the jasmonate co-receptor complex, inositol pentakisphosphate, which interacts with both COI1 and JAZ adjacent to the ligand. Our results unravel the mechanism of jasmonate perception and highlight the ability of F-box proteins to evolve as multi-component signalling hubs.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, Non-P.H.S.
MeSH terms
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Amino Acid Sequence
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Amino Acids / chemistry
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Amino Acids / metabolism
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Arabidopsis / chemistry
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Arabidopsis / metabolism
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Arabidopsis Proteins / chemistry*
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Arabidopsis Proteins / metabolism*
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Binding Sites
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Crystallography, X-Ray
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Cyclopentanes / chemistry
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Cyclopentanes / metabolism*
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F-Box Proteins / chemistry
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F-Box Proteins / metabolism
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Indenes / chemistry
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Indenes / metabolism
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Inositol Phosphates / metabolism*
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Isoleucine / analogs & derivatives
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Isoleucine / chemistry
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Isoleucine / metabolism
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Models, Molecular
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Molecular Sequence Data
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Oxylipins / chemistry
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Oxylipins / metabolism*
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Peptide Fragments / chemistry
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Peptide Fragments / metabolism
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Plant Growth Regulators / chemistry
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Plant Growth Regulators / metabolism*
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Protein Binding
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Protein Structure, Tertiary
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Repressor Proteins / chemistry*
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Repressor Proteins / metabolism*
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Signal Transduction
Substances
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ASK1 protein, Arabidopsis
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Amino Acids
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Arabidopsis Proteins
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COI1 protein, Arabidopsis
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Cyclopentanes
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F-Box Proteins
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Indenes
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Inositol Phosphates
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JAZ1 protein, Arabidopsis
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JAZ6 protein, Arabidopsis
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Oxylipins
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Peptide Fragments
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Plant Growth Regulators
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Repressor Proteins
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jasmonoyl-isoleucine
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Isoleucine
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inositol pentaphosphate
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coronatine
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jasmonic acid
Associated data
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PDB/3OGK
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PDB/3OGL
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PDB/3OGM