Localization capability and limitation of electron-multiplying charge-coupled, scientific complementary metal-oxide semiconductor, and charge-coupled devices for superresolution imaging

J Biomed Opt. 2010 Nov-Dec;15(6):066005. doi: 10.1117/1.3505017.

Abstract

Localization of a single fluorescent molecule is required in a number of superresolution imaging techniques for visualizing biological structures at cellular and subcellular levels. The localization capability and limitation of low-light detectors are critical for such a purpose. We present an updated evaluation on the performance of three typical low-light detectors, including a popular electron-multiplying CCD (EMCCD), a newly developed scientific CMOS (sCMOS), and a representative cooled CCD, for superresolution imaging. We find that under some experimental accessible conditions, the sCMOS camera shows a competitive and even better performance than the EMCCD camera, which has long been considered the detector of choice in the field of superresolution imaging.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amplifiers, Electronic*
  • Computer-Aided Design
  • Electron Transport
  • Equipment Design
  • Equipment Failure Analysis
  • Image Enhancement / instrumentation*
  • Metals / chemistry
  • Microscopy, Fluorescence / instrumentation*
  • Oxides / chemistry
  • Photometry / instrumentation*
  • Semiconductors*
  • Signal Processing, Computer-Assisted / instrumentation*
  • Static Electricity

Substances

  • Metals
  • Oxides