Abstract
Eukaryotes produce multiple products from a single gene locus by alternative splicing, translation or promoter usage as mechanisms expanding the complexity of their proteome. Trithorax proteins, including the Arabidopsis Trithorax-like protein ATX1, are histone modifiers regulating gene activity. Here, we report that a novel member of the Trithorax family has a role unrelated to chromatin. It is encoded from an internal promoter in the ATX1 locus as an isoform containing only the SET domain (soloSET). It is located exclusively in the cytoplasm and its substrate is the elongation factor 1A (EF1A). Loss of SET, but not of the histone modifying ATX1-SET activity, affects cytoskeletal actin bundling illustrating that the two isoforms have distinct functions in Arabidopsis cells.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, U.S. Gov't, Non-P.H.S.
MeSH terms
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Actin Cytoskeleton / ultrastructure
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Arabidopsis / genetics
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Arabidopsis / metabolism
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Arabidopsis / ultrastructure
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Arabidopsis Proteins / genetics*
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Arabidopsis Proteins / metabolism*
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Arabidopsis Proteins / physiology
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Cytoplasm / metabolism
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Histone-Lysine N-Methyltransferase
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Methylation
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Mutation
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Peptide Elongation Factor 1 / metabolism
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Promoter Regions, Genetic
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Protein Isoforms / genetics
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Protein Isoforms / metabolism
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Protein Isoforms / physiology
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Protein Structure, Tertiary
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Transcription Factors / genetics*
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Transcription Factors / metabolism*
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Transcription Factors / physiology
Substances
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Arabidopsis Proteins
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Peptide Elongation Factor 1
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Protein Isoforms
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Transcription Factors
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At2g31650 protein, Arabidopsis
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Histone-Lysine N-Methyltransferase