Construction of Pseudomonas syringae pv. tomato DC3000 mutant and polymutant strains

Brian H Kvitko; Alan Collmer

doi:10.1007/978-1-61737-998-7_10

Construction of Pseudomonas syringae pv. tomato DC3000 mutant and polymutant strains

Methods Mol Biol. 2011:712:109-28. doi: 10.1007/978-1-61737-998-7_10.

Authors

Brian H Kvitko¹, Alan Collmer

Affiliation

¹ Department of Plant Pathology and Plant-Microbe Biology, Cornell University, Ithaca, NY, USA.

PMID: 21359804
DOI: 10.1007/978-1-61737-998-7_10

Abstract

Redundancy between Pseudomonas syringae pv. tomato DC3000 virulence factors has made their characterization difficult. One method to circumvent redundancy for phenotypic characterization is to simultaneously delete all redundant factors through the generation of polymutant strains. Described here are methods by which single and polymutant strains of DC3000 can be generated through the use of the small mobilizable sucrose counter-selection vector pK18mobsacB, FRT-flanked antibiotic marker cassettes, and Flp recombination.

Publication types

Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

Animals
Gene Expression Regulation, Bacterial
Gene Targeting / methods*
Mutation*
Pseudomonas syringae / genetics*
Pseudomonas syringae / pathogenicity*
Solanum lycopersicum / microbiology*
Virulence Factors / genetics*

Substances

Virulence Factors