Simplified approach for quantitative digital holographic phase contrast imaging of living cells

J Biomed Opt. 2011 Feb;16(2):026014. doi: 10.1117/1.3540674.

Abstract

Many interferometry-based quantitative phase contrast imaging techniques require a separately generated coherent reference wave. This results in a low phase stability and the demand for a precise adjustment of the intensity ratio between object and reference wave. To overcome these problems, the performance of a Michelson interferometer approach for digital holographic microscopy was analyzed that avoids a separately generated reference wave by superposition of different image areas. It is shown that this simplified arrangement yields improved phase stability. Furthermore, results from time-lapse investigations on living pancreas tumor cells demonstrate the capability of the method for reliable quantitative phase contrast imaging.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cell Line, Tumor
  • Equipment Design
  • Equipment Failure Analysis
  • Holography / instrumentation*
  • Humans
  • Interferometry / instrumentation*
  • Microscopy, Phase-Contrast / instrumentation*
  • Pancreatic Neoplasms / pathology*
  • Reproducibility of Results
  • Sensitivity and Specificity
  • Signal Processing, Computer-Assisted / instrumentation*