Abstract
Hsc82 and Hsp82, the Hsp90 family proteins of yeast, are both required for fermentative growth at 37°C. Inactivation of either of the mitochondrial AAA proteases, Yme1 or Yta10/12, allows fermentative growth of hsc82∆ or hsp82∆ strains at 37°C. Genetic evidence indicates interaction of Hsc82/Hsp82 with the Yme1 and Yta10/Yta12 complexes in promoting F(1)F(o)-ATPase activity, with Hsc82 specifically required for F(1)-ATPase assembly. A previously reported mutation in Rpt3, one of the six ATPases of the proteasome, suppresses yme1∆ phenotypes and increases transcription of HSC82 but not HSP82. These genetic interactions describe a functional role for Hsp90 proteins in mitochondrial biogenesis.
© 2011 Elsevier B.V. and Mitochondria Research Society. All rights reserved.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, Non-U.S. Gov't
MeSH terms
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ATP-Dependent Proteases / genetics
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ATP-Dependent Proteases / metabolism*
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Adenosine Triphosphatases / genetics
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Adenosine Triphosphatases / metabolism*
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DNA, Mitochondrial
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Fermentation
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Gene Knockout Techniques
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Genotype
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HSP90 Heat-Shock Proteins / genetics
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HSP90 Heat-Shock Proteins / metabolism*
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Metalloendopeptidases / genetics
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Metalloendopeptidases / metabolism
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Mitochondria / genetics
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Mitochondria / metabolism
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Mitochondrial Proteins / genetics
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Mitochondrial Proteins / metabolism
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Mitochondrial Proton-Translocating ATPases / genetics
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Mitochondrial Proton-Translocating ATPases / metabolism*
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Mutation
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Saccharomyces cerevisiae / enzymology*
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Saccharomyces cerevisiae / genetics
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Saccharomyces cerevisiae / growth & development
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Saccharomyces cerevisiae Proteins / genetics
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Saccharomyces cerevisiae Proteins / metabolism*
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Temperature
Substances
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DNA, Mitochondrial
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HSP82 protein, S cerevisiae
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HSP90 Heat-Shock Proteins
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Mitochondrial Proteins
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Rpt3 protein, S cerevisiae
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Saccharomyces cerevisiae Proteins
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ATP-Dependent Proteases
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YME1 protein, S cerevisiae
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Metalloendopeptidases
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Adenosine Triphosphatases
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YTA12 protein, S cerevisiae
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AFG3 protein, S cerevisiae
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Mitochondrial Proton-Translocating ATPases