Effects of insulator cHS4 on transgene expression from plasmid DNA in a positive feedback system

J Biosci Bioeng. 2011 Nov;112(5):432-4. doi: 10.1016/j.jbiosc.2011.07.001. Epub 2011 Jul 29.

Abstract

The effects of a genetic insulator (chicken cHS4) on transgene expression in a positive feedback system using the GAL4-VP16 activator and luciferase reporter plasmids were examined. The introduction of cHS4 enhanced luciferase expression in mouse liver, indicating that this system would be useful for efficient transgene expression.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cell Line
  • DNA / genetics
  • Female
  • Gene Expression Regulation
  • Genetic Vectors
  • Insulator Elements*
  • Luciferases / genetics
  • Mice
  • Mice, Inbred BALB C
  • Mice, Transgenic
  • Plasmids
  • Trans-Activators / genetics
  • Transgenes*

Substances

  • Gal-VP16
  • Trans-Activators
  • DNA
  • Luciferases