A two component system is involved in acid adaptation of Lactobacillus delbrueckii subsp. bulgaricus

Yanhua Cui; Wei Liu; Xiaojun Qu; Zhangting Chen; Xu Zhang; Tong Liu; Lanwei Zhang

doi:10.1016/j.micres.2011.11.003

A two component system is involved in acid adaptation of Lactobacillus delbrueckii subsp. bulgaricus

Microbiol Res. 2012 May 20;167(5):253-61. doi: 10.1016/j.micres.2011.11.003. Epub 2011 Dec 15.

Authors

Yanhua Cui¹, Wei Liu, Xiaojun Qu, Zhangting Chen, Xu Zhang, Tong Liu, Lanwei Zhang

Affiliation

¹ School of Food Science and Engineering, Harbin Institute of Technology, Harbin 150090, PR China. yhcui@hit.edu.cn

PMID: 22177749
DOI: 10.1016/j.micres.2011.11.003

Abstract

The Gram-positive bacterium Lactobacillus delbrueckii subsp. bulgaricus is of vital importance to the food industry, especially to the dairy industry. Two component systems (TCSs) are one of the most important mechanisms for environmental sensing and signal transduction in the majority of Gram-positive and Gram-negative bacteria. A typical TCS consists of a histidine protein kinase (HPK) and a cytoplasmic response regulator (RR). To investigate the functions of TCSs during acid adaptation in L. bulgaricus, we used quantitative PCR to reveal how TCSs expression changes during acid adaptation. Two TCSs (JN675228/JN675229 and JN675230/JN675231) and two HPKs (JN675236 and JN675240) were induced during acid adaptation. These TCSs were speculated to be related with the acid adaptation ability of L. bulgaricus. The mutants of JN675228/JN675229 were constructed in order to investigate the functions of JN675228/JN675229. The mutants showed reduced acid adaptation compared to that of wild type, and the complemented strains were similar to the wild-type strain. These observations suggested that JN675228 and JN675229 were involved in acid adaptation in L. bulgaricus. The interaction between JN675228 and JN675229 was identified by means of yeast two-hybrid system. The results indicated there is interaction between JN675228 and JN675229.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Acids / metabolism*
Adaptation, Physiological
Bacterial Proteins / genetics
Bacterial Proteins / metabolism*
Gene Expression Regulation, Bacterial
Histidine Kinase
Lactobacillus delbrueckii / enzymology
Lactobacillus delbrueckii / genetics
Lactobacillus delbrueckii / physiology*
Protein Binding
Protein Kinases / genetics
Protein Kinases / metabolism*

Substances

Acids
Bacterial Proteins
Protein Kinases
Histidine Kinase