Abstract
The half-life times of photosystem I and II proteins were determined using (15)N-labeling and mass spectrometry. The half-life times (30-75h for photosystem I components and <1-11h for the large photosystem II proteins) were similar when proteins were isolated from monomeric vs. oligomeric complexes on Blue-Native gels, suggesting that the two forms of both photosystems can interchange on a timescale of <1h or that only one form of each photosystem exists in thylakoids in vivo. The half-life times of proteins associated with either photosystem generally were unaffected by the absence of Small Cab-like proteins.
Copyright © 2011 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.
Publication types
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Research Support, U.S. Gov't, Non-P.H.S.
MeSH terms
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Cells, Cultured
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Cyanobacteria / enzymology
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Cyanobacteria / genetics
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Cyanobacteria / metabolism
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Half-Life
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Multiprotein Complexes / chemistry
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Multiprotein Complexes / metabolism
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Photosynthetic Reaction Center Complex Proteins / chemistry
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Photosynthetic Reaction Center Complex Proteins / metabolism
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Photosystem I Protein Complex / genetics
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Photosystem I Protein Complex / metabolism*
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Photosystem I Protein Complex / physiology
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Photosystem II Protein Complex / genetics
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Photosystem II Protein Complex / metabolism*
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Photosystem II Protein Complex / physiology
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Proteolysis*
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Synechocystis / enzymology
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Synechocystis / genetics
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Synechocystis / metabolism*
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Time Factors
Substances
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Multiprotein Complexes
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Photosynthetic Reaction Center Complex Proteins
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Photosystem I Protein Complex
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Photosystem II Protein Complex