Abstract
A derivative of N(ε)-benzyloxycarbonyl-L-lysine with a photo-reactive diazirinyl group, N(ε)-[((4-(3-(trifluoromethyl)-3H-diazirin-3-yl)benzyl)oxy)carbonyl]-L-lysine, was site-specifically incorporated into target proteins in mammalian cells. The incorporated photo-crosslinker is able to react not only with residues as distant as about 15 Å but also with those in closer proximity, thus enabling "wide-range" photo-crosslinking of proteins.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Adaptor Proteins, Signal Transducing / genetics
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Adaptor Proteins, Signal Transducing / metabolism
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Animals
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Azirines / chemistry*
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Benzophenones / chemistry
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CHO Cells
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Cricetinae
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Cross-Linking Reagents
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HEK293 Cells
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Humans
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Lysine / analogs & derivatives*
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Lysine / chemistry
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Phenylalanine / analogs & derivatives*
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Phenylalanine / chemistry
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Protein Conformation
Substances
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4-benzoylphenylalanine
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Adaptor Proteins, Signal Transducing
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Azirines
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Benzophenones
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Cross-Linking Reagents
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N(epsilon)-(benzyloxycarbonyl)lysine
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Phenylalanine
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3-(4-(3-(trifluoromethyl)-3H-diazirin-3-yl)phenyl)alanine
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Lysine