Abstract
Pannexin 1 (Panx1) is expressed in various mammalian tissues including the brain and immune cells. Here, we present evidence that Panx1 when expressed in mammalian cells, forms anion-selective channels, with a rank order of permeabilities: NO (3) (-)> I(-) > Br (-)> Cl (-) > F (-)>> aspartate (-)≈ glutamate (-)≈ gluconate(-). Single-channel Panx1-mediated currents have a unitary conductance around 68 pS. Our results show that Panx1 assembles into a membrane anion channel with a relatively low single-channel conductance.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Anions / metabolism*
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Aspartic Acid / metabolism
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Cell Line
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Cell Membrane Permeability / physiology*
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Chlorides / metabolism
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Connexins / metabolism*
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Glutamic Acid / metabolism
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HEK293 Cells
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Humans
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Ion Channels / metabolism
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Nerve Tissue Proteins / metabolism*
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Voltage-Dependent Anion Channels / metabolism*
Substances
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Anions
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Chlorides
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Connexins
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Ion Channels
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Nerve Tissue Proteins
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PANX1 protein, human
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Voltage-Dependent Anion Channels
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Aspartic Acid
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Glutamic Acid