PIWI associated siRNAs and piRNAs specifically require the Caenorhabditis elegans HEN1 ortholog henn-1

PLoS Genet. 2012;8(4):e1002616. doi: 10.1371/journal.pgen.1002616. Epub 2012 Apr 19.

Abstract

Small RNAs--including piRNAs, miRNAs, and endogenous siRNAs--bind Argonaute proteins to form RNA silencing complexes that target coding genes, transposons, and aberrant RNAs. To assess the requirements for endogenous siRNA formation and activity in Caenorhabditis elegans, we developed a GFP-based sensor for the endogenous siRNA 22G siR-1, one of a set of abundant siRNAs processed from a precursor RNA mapping to the X chromosome, the X-cluster. Silencing of the sensor is also dependent on the partially complementary, unlinked 26G siR-O7 siRNA. We show that 26G siR-O7 acts in trans to initiate 22G siRNA formation from the X-cluster. The presence of several mispairs between 26G siR-O7 and the X-cluster mRNA, as well as mutagenesis of the siRNA sensor, indicates that siRNA target recognition is permissive to a degree of mispairing. From a candidate reverse genetic screen, we identified several factors required for 22G siR-1 activity, including the chromatin factors mes-4 and gfl-1, the Argonaute ergo-1, and the 3' methyltransferase henn-1. Quantitative RT-PCR of small RNAs in a henn-1 mutant and deep sequencing of methylated small RNAs indicate that siRNAs and piRNAs that associate with PIWI clade Argonautes are methylated by HENN-1, while siRNAs and miRNAs that associate with non-PIWI clade Argonautes are not. Thus, PIWI-class Argonaute proteins are specifically adapted to associate with methylated small RNAs in C. elegans.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Animals
  • Animals, Genetically Modified
  • Argonaute Proteins* / genetics
  • Argonaute Proteins* / metabolism
  • Biosensing Techniques / methods
  • Caenorhabditis elegans Proteins / genetics*
  • Caenorhabditis elegans Proteins / metabolism
  • Caenorhabditis elegans* / genetics
  • Caenorhabditis elegans* / growth & development
  • High-Throughput Nucleotide Sequencing
  • Methylation
  • Mutation
  • Nerve Tissue Proteins / genetics*
  • Nerve Tissue Proteins / metabolism
  • Phylogeny
  • RNA Interference
  • RNA, Small Interfering / genetics*
  • RNA, Small Interfering / metabolism
  • RNA-Binding Proteins / genetics
  • X Chromosome / genetics

Substances

  • Argonaute Proteins
  • Caenorhabditis elegans Proteins
  • ERGO-1 protein, C elegans
  • HEN-1 protein, C elegans
  • Mes-4 protein, C elegans
  • Nerve Tissue Proteins
  • RNA, Small Interfering
  • RNA-Binding Proteins

Associated data

  • GEO/GSE35550