Impact of normalization method on experimental outcome using RT-qPCR in Staphylococcus aureus

Lukas Valihrach; Katerina Demnerova

doi:10.1016/j.mimet.2012.05.008

Impact of normalization method on experimental outcome using RT-qPCR in Staphylococcus aureus

J Microbiol Methods. 2012 Sep;90(3):214-6. doi: 10.1016/j.mimet.2012.05.008. Epub 2012 May 18.

Authors

Lukas Valihrach¹, Katerina Demnerova

Affiliation

¹ Institute of Chemical Technology, Prague, Faculty of Food and Biochemical Technology, Department of Biochemistry and Microbiology, Prague, Czech Republic. valihral@vscht.cz

PMID: 22613804
DOI: 10.1016/j.mimet.2012.05.008

Abstract

We performed a gene expression study using RT-qPCR in Staphylococcus aureus. The influence of normalization method was investigated. We confirmed that a recent standard, using more reference genes, was the best normalization strategy. The application of the most commonly used reference genes in 2011 (gyrB and 16S rRNA gene) failed.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

DNA Gyrase / genetics
Gene Expression
Genes, Bacterial*
RNA, Bacterial / genetics
RNA, Ribosomal, 16S / genetics
Real-Time Polymerase Chain Reaction / standards*
Reference Standards
Reverse Transcriptase Polymerase Chain Reaction / standards*
Staphylococcus aureus / genetics*
Transcriptome

Substances

RNA, Bacterial
RNA, Ribosomal, 16S
DNA Gyrase