Catalyst-free and site-specific one-pot dual-labeling of a protein directed by two genetically incorporated noncanonical amino acids

Chembiochem. 2012 Jul 9;13(10):1405-8. doi: 10.1002/cbic.201200281. Epub 2012 May 24.

Abstract

The genetic incorporation of one azide-containing and one keto-containing noncanonical amino acids into a protein at one amber and one ochre mutation sites respectively followed by their orthogonal reactions with one hydroxylamine-containing and one cyclooctyne-containing dyes allows highly efficient one-pot site-specific dual labeling of the protein in a catalyst-free fashion.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Alkynes / chemistry
  • Amino Acids / chemistry
  • Amino Acids / metabolism*
  • Azides / chemistry
  • Carrier Proteins / chemistry
  • Carrier Proteins / genetics
  • Carrier Proteins / metabolism
  • Catalysis
  • Click Chemistry
  • Fluorescence Resonance Energy Transfer
  • Proteins / chemistry
  • Proteins / genetics
  • Proteins / metabolism*

Substances

  • Alkynes
  • Amino Acids
  • Azides
  • Carrier Proteins
  • Proteins
  • glutamine transport proteins