Abstract
Anatomy of large biological specimens is often reconstructed from serially sectioned volumes imaged by high-resolution microscopy. We developed a method to reassemble a continuous volume from such large section series that explicitly minimizes artificial deformation by applying a global elastic constraint. We demonstrate our method on a series of transmission electron microscopy sections covering the entire 558-cell Caenorhabditis elegans embryo and a segment of the Drosophila melanogaster larval ventral nerve cord.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Caenorhabditis elegans / ultrastructure
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Drosophila melanogaster / ultrastructure
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Elasticity
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Embryo, Nonmammalian / ultrastructure
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Image Processing, Computer-Assisted / instrumentation
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Image Processing, Computer-Assisted / methods*
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Larva / ultrastructure
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Microscopy, Electron, Transmission / instrumentation
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Microscopy, Electron, Transmission / methods*
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Microtomy / instrumentation
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Microtomy / methods*
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Pattern Recognition, Automated / methods*