Abstract
To assess the clonal structure of Staphylococcus aureus in the United States, we performed a molecular epidemiological study of 1,055 S. aureus isolates from a nationally representative clinical isolate collection from 2004-2008. Resistant and susceptible isolates were typed with multilocus sequence typing, tested for the presence of Panton-Valentine leukocidin (PVL), and serotyped. USA300 (multilocus sequence typing clonal complex 8, PVL positive, and methicillin-resistant) was the most frequently isolated clone, expanding from 12% of all isolates in 2004 to 38% in 2006. The USA300 clone increased significantly in frequency among both outpatients and inpatients. USA300 increased in both skin and soft-tissue and invasive infection isolates. The second most frequently observed clone was clonal complex 5, PVL-negative, and methicillin-resistant, and its frequency was stable from 2004-2008. The methicillin-susceptible S. aureus in the study was polyclonal, and decreased in frequency as it was replaced by USA300.
Publication types
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Adolescent
-
Adult
-
Aged
-
Aged, 80 and over
-
Anti-Bacterial Agents / pharmacology
-
Anti-Bacterial Agents / therapeutic use
-
Bacterial Toxins / analysis
-
Child
-
Child, Preschool
-
Clone Cells
-
Exotoxins / analysis
-
Female
-
Humans
-
Incidence
-
Infant
-
Infant, Newborn
-
Inpatients
-
Leukocidins / analysis
-
Male
-
Methicillin Resistance / genetics
-
Middle Aged
-
Multilocus Sequence Typing
-
Outpatients
-
Retrospective Studies
-
Serotyping
-
Skin / drug effects
-
Skin / microbiology
-
Soft Tissue Infections / drug therapy
-
Soft Tissue Infections / epidemiology*
-
Soft Tissue Infections / microbiology
-
Staphylococcal Infections / drug therapy
-
Staphylococcal Infections / epidemiology*
-
Staphylococcal Infections / microbiology
-
Staphylococcal Skin Infections / drug therapy
-
Staphylococcal Skin Infections / epidemiology*
-
Staphylococcal Skin Infections / microbiology
-
Staphylococcus aureus / drug effects
-
Staphylococcus aureus / genetics*
-
Staphylococcus aureus / isolation & purification
-
United States / epidemiology
Substances
-
Anti-Bacterial Agents
-
Bacterial Toxins
-
Exotoxins
-
Leukocidins
-
Panton-Valentine leukocidin