Genetically targetable and color-switching fluorescent probe

Chembiochem. 2012 Jul 23;13(11):1564-8. doi: 10.1002/cbic.201200334. Epub 2012 Jul 6.

Abstract

Color bind: We have developed a probe TMR-para-MG that switches its fluorescence emission upon binding to a fluorogen-activating protein (FAP). In cells that express FAP, this dye labels target sites in one color and mitochondria in another color, thus it might be a suitable tool for monitoring changes in mitochondrial membrane potential.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Color*
  • Fluorescence Resonance Energy Transfer
  • Fluorescent Dyes / chemical synthesis
  • Fluorescent Dyes / chemistry*
  • Fluorescent Dyes / metabolism
  • HeLa Cells
  • Humans
  • Membrane Potential, Mitochondrial
  • Membrane Proteins / chemistry*
  • Membrane Proteins / genetics*
  • Membrane Proteins / metabolism
  • Mitochondria / metabolism
  • Models, Molecular
  • Molecular Structure
  • Rhodamines / chemistry*
  • Rhodamines / metabolism
  • Rosaniline Dyes / chemical synthesis
  • Rosaniline Dyes / chemistry*
  • Rosaniline Dyes / metabolism
  • Spectrometry, Fluorescence

Substances

  • Fluorescent Dyes
  • Membrane Proteins
  • Rhodamines
  • Rosaniline Dyes
  • malachite green
  • tetramethylrhodamine