RBMX: a regulator for maintenance and centromeric protection of sister chromatid cohesion

Sachihiro Matsunaga; Hideaki Takata; Akihiro Morimoto; Kayoko Hayashihara; Tsunehito Higashi; Kouhei Akatsuchi; Eri Mizusawa; Mariko Yamakawa; Mamoru Ashida; Tomoko M Matsunaga; Takachika Azuma; Susumu Uchiyama; Kiichi Fukui

doi:10.1016/j.celrep.2012.02.005

RBMX: a regulator for maintenance and centromeric protection of sister chromatid cohesion

Cell Rep. 2012 Apr 19;1(4):299-308. doi: 10.1016/j.celrep.2012.02.005. Epub 2012 Mar 22.

Authors

Sachihiro Matsunaga¹, Hideaki Takata, Akihiro Morimoto, Kayoko Hayashihara, Tsunehito Higashi, Kouhei Akatsuchi, Eri Mizusawa, Mariko Yamakawa, Mamoru Ashida, Tomoko M Matsunaga, Takachika Azuma, Susumu Uchiyama, Kiichi Fukui

Affiliation

¹ Department of Biotechnology, Graduate School of Engineering, Osaka University, Suita 565-0871, Japan. sachi@rs.tus.ac.jp

PMID: 22832223
DOI: 10.1016/j.celrep.2012.02.005

Abstract

Cohesion is essential for the identification of sister chromatids and for the biorientation of chromosomes until their segregation. Here, we have demonstrated that an RNA-binding motif protein encoded on the X chromosome (RBMX) plays an essential role in chromosome morphogenesis through its association with chromatin, but not with RNA. Depletion of RBMX by RNA interference (RNAi) causes the loss of cohesin from the centromeric regions before anaphase, resulting in premature chromatid separation accompanied by delocalization of the shugoshin complex and outer kinetochore proteins. Cohesion defects caused by RBMX depletion can be detected as early as the G2 phase. Moreover, RBMX associates with the cohesin subunits, Scc1 and Smc3, and with the cohesion regulator, Wapl. RBMX is required for cohesion only in the presence of Wapl, suggesting that RBMX is an inhibitor of Wapl. We propose that RBMX is a cohesion regulator that maintains the proper cohesion of sister chromatids.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Carrier Proteins / metabolism
Carrier Proteins / physiology
Cell Cycle Proteins / metabolism
Cell Cycle Proteins / physiology
Centromere / metabolism
Chondroitin Sulfate Proteoglycans / metabolism
Chondroitin Sulfate Proteoglycans / physiology
Chromatids / metabolism
Chromatids / physiology*
Chromosomal Proteins, Non-Histone / metabolism
Chromosomal Proteins, Non-Histone / physiology
DNA-Binding Proteins
G2 Phase
HeLa Cells
Heterogeneous-Nuclear Ribonucleoproteins / genetics
Heterogeneous-Nuclear Ribonucleoproteins / metabolism
Heterogeneous-Nuclear Ribonucleoproteins / physiology*
Humans
Nuclear Proteins / metabolism
Nuclear Proteins / physiology
Phosphoproteins / metabolism
Phosphoproteins / physiology
Proto-Oncogene Proteins / metabolism
Proto-Oncogene Proteins / physiology
RNA Interference

Substances

Carrier Proteins
Cell Cycle Proteins
Chondroitin Sulfate Proteoglycans
Chromosomal Proteins, Non-Histone
DNA-Binding Proteins
Heterogeneous-Nuclear Ribonucleoproteins
Nuclear Proteins
Phosphoproteins
Proto-Oncogene Proteins
RAD21 protein, human
RBMX protein, human
SMC3 protein, human
WAPL protein, human