Genetic variants in REC8, RNF212, and PRDM9 influence male recombination in cattle

Cynthia Sandor; Wanbo Li; Wouter Coppieters; Tom Druet; Carole Charlier; Michel Georges

doi:10.1371/journal.pgen.1002854

Genetic variants in REC8, RNF212, and PRDM9 influence male recombination in cattle

PLoS Genet. 2012;8(7):e1002854. doi: 10.1371/journal.pgen.1002854. Epub 2012 Jul 26.

Authors

Cynthia Sandor¹, Wanbo Li, Wouter Coppieters, Tom Druet, Carole Charlier, Michel Georges

Affiliation

¹ Unit of Animal Genomics, GIGA-R and Faculty of Veterinary Medicine, University of Liège, Liège, Belgium.

Abstract

We use >250,000 cross-over events identified in >10,000 bovine sperm cells to perform an extensive characterization of meiotic recombination in male cattle. We map Quantitative Trait Loci (QTL) influencing genome-wide recombination rate, genome-wide hotspot usage, and locus-specific recombination rate. We fine-map three QTL and present strong evidence that genetic variants in REC8 and RNF212 influence genome-wide recombination rate, while genetic variants in PRDM9 influence genome-wide hotspot usage.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Cattle
Cell Cycle Proteins / genetics
Chromosome Mapping
Crossing Over, Genetic*
Genetic Linkage
Genome
Histone-Lysine N-Methyltransferase / genetics
Homologous Recombination / genetics*
Humans
Ligases
Male
Miosis / genetics
Pedigree
Polymorphism, Single Nucleotide
Quantitative Trait Loci / genetics*
Sequence Homology, Amino Acid*
Sperm Count
Spermatozoa / cytology*
Ubiquitin-Protein Ligases / genetics

Substances

Cell Cycle Proteins
REC8 protein, human
Histone-Lysine N-Methyltransferase
PRDM9 protein, human
Ubiquitin-Protein Ligases
Ligases
RNF212 protein, human