Monoclonal antibodies directed against the calcium binding protein Calbindin D-28k

M R Celio; W Baier; L Schärer; H J Gregersen; P A de Viragh; A W Norman

doi:10.1016/0143-4160(90)90014-l

Monoclonal antibodies directed against the calcium binding protein Calbindin D-28k

Cell Calcium. 1990 Oct;11(9):599-602. doi: 10.1016/0143-4160(90)90014-l.

Authors

M R Celio¹, W Baier, L Schärer, H J Gregersen, P A de Viragh, A W Norman

Affiliation

¹ Institute of Histology, University of Fribourg, Switzerland.

PMID: 2285928
DOI: 10.1016/0143-4160(90)90014-l

Abstract

We have produced 25 clones secreting antibodies directed against chicken Calbindin D-28k. Two of them, 300 and 318, recognize determinants conserved in fish, chicken, mouse, rat, rabbit, monkey and human Calbindin D-28k. We demonstrate their use in the immunohistochemical localization of Calbindin D-28k, and in the detection of Calbindin D-28k on immunoblots.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Antibodies, Monoclonal / immunology*
Binding, Competitive
Calbindins
Chickens
Cross Reactions
Humans
Immunoblotting
Immunohistochemistry
Rats
S100 Calcium Binding Protein G / analysis
S100 Calcium Binding Protein G / immunology*
S100 Calcium Binding Protein G / metabolism

Substances

Antibodies, Monoclonal
Calbindins
S100 Calcium Binding Protein G