Abstract
Phospholipase C produces two second messengers--diacylglycerol (DAG), which remains in the membrane, and inositol triphosphate (IP(3)), which triggers the release of calcium ions (Ca(2+)) from intracellular stores. Genetically encoded sensors based on a single circularly permuted fluorescent protein (FP) are robust tools for studying intracellular Ca(2+) dynamics. We have developed a robust sensor for DAG based on a circularly permuted green FP that can be co-imaged with the red fluorescent Ca(2+) sensor R-GECO for simultaneous measurement of both second messengers.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, Non-U.S. Gov't
MeSH terms
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Biosensing Techniques / methods*
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Calcium / metabolism*
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Cell Survival
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Diglycerides / metabolism*
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Fluorescent Dyes / metabolism
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HEK293 Cells
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Humans
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Intracellular Space / metabolism
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Luminescent Proteins / chemistry
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Luminescent Proteins / metabolism
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Protein Conformation
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Protein Kinase C-delta / chemistry
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Protein Kinase C-delta / metabolism
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Second Messenger Systems*
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Time Factors
Substances
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Diglycerides
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Fluorescent Dyes
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Luminescent Proteins
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Protein Kinase C-delta
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Calcium