Abstract
Fibroblast growth factor18 (FGF18) belongs to the FGF family and is a pleiotropic protein that stimulates proliferation in several tissues. Bone marrow mesenchymal stem cells (BMSCs) participate in the normal replacement of damaged cells and in disease healing processes within bone and the haematopoietic system. In this study, we constructed FGF18 and investigated its effects on rat BMSCs (rBMSCs). The proliferative effects of FGF18 on rBMSCs were examined using an MTS assay. To validate the osteogenic differentiation effects of FGF18, ALP and mineralization activity were examined as well as osteogenic differentiation-related gene levels. FGF18 significantly enhanced rBMSCs proliferation (p<0.001) and induced the osteogenic differentiation by elevating ALP and mineralization activity of rBMSCs (p<0.001). Furthermore, these osteogenic differentiation effects of FGF18 were confirmed via increasing the mRNA levels of collagen type I (Col I), bone morphogenetic protein 4 (BMP4), and Runt-related transcription factor 2 (Runx2) at 3 and 7 days. These results suggest that FGF18 could be used to improve bone repair and regeneration.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, Non-U.S. Gov't
MeSH terms
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Alkaline Phosphatase / metabolism
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Animals
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Bone Marrow Cells / cytology*
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Bone Marrow Cells / drug effects
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Bone Marrow Cells / physiology
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Bone Morphogenetic Protein 4 / metabolism
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Cell Differentiation / drug effects
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Cell Differentiation / physiology*
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Cell Proliferation / drug effects
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Cells, Cultured
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Collagen Type I / metabolism
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Core Binding Factor Alpha 1 Subunit / metabolism
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Fibroblast Growth Factors / metabolism*
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Mesenchymal Stem Cells / cytology*
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Mesenchymal Stem Cells / drug effects
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Mesenchymal Stem Cells / physiology
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Osteogenesis / drug effects
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Osteogenesis / physiology*
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Rats
Substances
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Bmp4 protein, rat
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Bone Morphogenetic Protein 4
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Collagen Type I
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Core Binding Factor Alpha 1 Subunit
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Runx2 protein, rat
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fibroblast growth factor 18
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Fibroblast Growth Factors
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Alkaline Phosphatase
Grants and funding
This work was supported by Priority Research Centers Program (grant#: 2009-0093829) and WCU (World Class University) program (grant#: R31-10069) and Basic Science Research program through the National Research Foundation (NRF) funded by the Ministry of Education, Science and Technology (grant#: 2010-0022628) through the National Research Foundation (NRF) funded by the Ministry of Education, Science and Technology and a grant of the Korea Healthcare technology R&D Project, Ministry of Health & Welfare, Republic of Korea (A110416) and Inha University Grant. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.