Abstract
Escherichia coli possesses a number of proteins that transport sugars out of the cell. We identified 31 candidate sugar efflux transporters based on their similarity to known sugar efflux transporters. We then tested whether these transporters affect arabinose and xylose metabolism. We identified 13 transporters - setC, cmr, ynfM, mdtD, yfcJ, yhhS, emrD, ydhC, ydeA, ybdA, ydeE, mhpT, and kgtP - that appeared to increase or decrease intracellular arabinose concentrations when respectively deleted or over-expressed. None of the candidate transporters affected xylose concentrations. These results indicate that E. coli possesses multiple arabinose efflux transporters. They also provide a novel target for future metabolic engineering.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Arabinose / metabolism*
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Computational Biology*
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Escherichia coli / genetics
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Escherichia coli / metabolism*
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Escherichia coli Proteins / genetics
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Escherichia coli Proteins / metabolism*
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Monosaccharide Transport Proteins / deficiency
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Monosaccharide Transport Proteins / genetics
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Monosaccharide Transport Proteins / metabolism*
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Sequence Deletion
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Sequence Homology, Nucleic Acid
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Xylose / metabolism*
Substances
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Escherichia coli Proteins
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Monosaccharide Transport Proteins
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Xylose
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Arabinose
Grants and funding
This work was funded by the Energy Biosciences Institute. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.