Ultraviolet irradiation-dependent fluorescence enhancement of hemoglobin catalyzed by reactive oxygen species

Leiting Pan; Xiaoxu Wang; Shuying Yang; Xian Wu; Imshik Lee; Xinzheng Zhang; Romano A Rupp; Jingjun Xu

doi:10.1371/journal.pone.0044142

Ultraviolet irradiation-dependent fluorescence enhancement of hemoglobin catalyzed by reactive oxygen species

PLoS One. 2012;7(8):e44142. doi: 10.1371/journal.pone.0044142. Epub 2012 Aug 30.

Authors

Leiting Pan¹, Xiaoxu Wang, Shuying Yang, Xian Wu, Imshik Lee, Xinzheng Zhang, Romano A Rupp, Jingjun Xu

Affiliation

¹ The Key Laboratory of Weak-Light Nonlinear Photonics, Ministry of Education, TEDA Applied Physics School and School of Physics, Nankai University, Tianjin, China. plt@nankai.edu.cn

Abstract

Ultraviolet (UV) light has a potent effect on biological organisms. Hemoglobin, an oxygen-transport protein, plays an irreplaceable role in sustaining life of all vertebrates. In this study we scrutinize the effects of ultraviolet irradiation (UVI) as well as visible irradiation on the fluorescence characteristics of bovine hemoglobin (BHb) in vitro. Data show that UVI results in fluorescence enhancement of BHb in a dose-dependent manner. Furthermore, UVI-induced fluorescence enhancement is significantly increased when BHb is pretreated with hydrogen peroxide (H(2)O(2)), a type of reactive oxygen species (ROS). Meanwhile, The water-soluble antioxidant vitamin C suppresses this UVI-induced fluorescence enhancement. In contrast, green light irradiation does not lead to fluorescence enhancement of BHb no matter whether H(2)O(2) is acting on the BHb solution or not. Taken together, these results indicate that catalysis of ROS and UVI-dependent irradiation play two key roles in the process of UVI-induced fluorescence enhancement of BHb.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Absorption
Animals
Ascorbic Acid / pharmacology
Biocatalysis / drug effects*
Biocatalysis / radiation effects*
Cattle
Dose-Response Relationship, Radiation
Fluorescence
Hemoglobins / metabolism*
Hemoglobins / radiation effects*
Hydrogen Peroxide / pharmacology
Protective Agents / pharmacology
Reactive Oxygen Species / pharmacology*
Spectrophotometry, Ultraviolet
Ultraviolet Rays*

Substances

Hemoglobins
Protective Agents
Reactive Oxygen Species
Hydrogen Peroxide
Ascorbic Acid

Grants and funding

This work was supported by the Specialized Research Fund for the Doctoral Program of Higher Education (New Teachers) (No. 20110031120004), the Fundamental Research Funds for the Central Universities, the National Natural Science Foundation of China (No. 11074133), the National Basic Research Program of China (Nos. 2007CB307002, 2010CB934101), 111 Project (No. B07013) and International S&T cooperation program of China (2011DFA52870). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.