Natural deletion of L35Y36 in p6 gag eliminate LYPXnL/ALIX auxiliary virus release pathway in HIV-1 subtype C

Ajit Patil; Jayanta Bhattacharya

doi:10.1016/j.virusres.2012.08.020

Natural deletion of L35Y36 in p6 gag eliminate LYPXnL/ALIX auxiliary virus release pathway in HIV-1 subtype C

Virus Res. 2012 Dec;170(1-2):154-8. doi: 10.1016/j.virusres.2012.08.020. Epub 2012 Sep 4.

Authors

Ajit Patil¹, Jayanta Bhattacharya

Affiliation

¹ Department of Molecular Virology, National AIDS Research Institute, G-73 MIDC, Bhosari, Pune, India.

PMID: 22981647
DOI: 10.1016/j.virusres.2012.08.020

Abstract

Natural loss of L35Y36 residues in ALIX binding site of HIV-1 subtype C was found to prevent the p6 gag-ALIX interaction. Over expression of ALIX 364-716 (V-domain) unlike pNL4.3 (subtype B), also did not inhibit the release of chimeric pNL4.3 expressing subtype C p6 late domain. Loss of V domain binding consequently affected the ALIX mediated particle release in the absence of PTAP/TSG101 pathway. Our data indicated absence of LYPXnL/ALIX pathway in HIV-1 subtype C.

Publication types

Research Support, N.I.H., Extramural

MeSH terms

Amino Acid Motifs*
Amino Acid Sequence
Gene Order
HIV-1 / classification
HIV-1 / physiology*
Humans
Molecular Sequence Data
Sequence Alignment
Sequence Deletion*
Virus Release* / genetics
Virus Replication
gag Gene Products, Human Immunodeficiency Virus / chemistry*
gag Gene Products, Human Immunodeficiency Virus / genetics*

Substances

gag Gene Products, Human Immunodeficiency Virus
p6 gag protein, Human immunodeficiency virus 1